Program 01FramingUpdated 05 Sep 2026

Engineer the receptor, locus, and cell as one system.

TCRForge turns a T-cell engineering question into an auditable chain of locus biology, construct choices, model evidence, experiments, and decisions.

Decision frame

What must be true before this construct advances?

TF-P01 · v0.2

Research questionDoes TRAC-targeted integration preserve useful expression control while reducing receptor competition?

Cell contextPrimary human T cellsActivation and manufacturing state pending

Current gateArchitecture selectionBefore donor and assay lock

Select an element to inspect the evidence boundary.

Established Context-dependent Unresolved

Selected element

A clinically important integration site for coupling receptor payloads to endogenous control.

Established

Compare complete cell systems, not isolated cassettes.

Candidate systemExpression controlReview stateNext discriminating evidence

TRAC homology-directed knock-inEndogenous locusLead architectureResolve donor geometry and junction assay

Safe-harbor, promoter-driven cassetteSynthetic promoterComparatorBenchmark expression variance and exhaustion

Lentiviral reference constructVector copy dependentBaselineLock manufacturing and copy-number assumptions

5-axis program view

42%

Locus regulation68%

Construct geometry46%

Specificity & function34%

Genome safety27%

Cell phenotype35%

Claims requiring resolution

Regulation

Eα and Tα2 evidence cannot be transferred across cell and construct contexts without qualification.

Genome integrity

On-target junction success is not enough to rule out structural variants or unintended integration.

Translation

It creates a concrete bridge between locus biology, construct design, editing and cell phenotype.

Next decision gate

TRAC knock-in vs safe-harbor vs lentiviral baseline, evaluated under the same cell and assay context.